Довольно часто на гинекологическом осмотре врач выявляет реактивные изменения эпителия шейки матки, о существовании которых женщина может и не знать. При этом по статистике патологические процессы в этой области можно обнаружить у женщин репродуктивного возраста от 21 до 27 лет.
Шейка матки в норме
Когда шейка матки в норме, то она имеет форму цилиндра или усеченного конуса и равномерный розовый окрас. Ее покрывает слой эпителия, который изменяется при наличии малейших отклонений. От состояния шейки матки зависит успех беременности и родов. Реактивные изменения плоского эпителия шейки матки можно выявить при проведении пробы Шиллера и иных исследований.
Диагностика и причины
Причинами появления реактивных изменений клеток эпителия шейки матки могут выступать возбудители различных заболеваний, передающихся половым путем, а также представители естественной микрофлоры полового тракта, состав, которых меняется при понижении иммунитета женщины.
Признаками реактивных изменений являются:
- покраснение слизистой оболочки
- рост лейкоцитов
- рост эпителиальных клеток (их можно обнаружить в мазке на микрофлору влагалища)
Сама женщина может обнаружить неприятный запах и изменившийся характер выделений из влагалища. Явными признаками патологического состояния считаются кровяные выделения, появляющиеся после полового акта.
Обнаружить дефект на шейке матки гинеколог может при осмотре на кресле с использованием кольпоскопа. Данное исследование должно проводиться независимо от состояния женщины раз в полгода. Его целью является выявление только начавшихся патологических изменений. Для подтверждения диагноза назначаются дополнительно такие исследования, как проба Шиллера и соскоб на онкоцитологию.
Стоит сразу отметить, что реактивные изменения эпителия шейки матки нестрашны и легко обратимы при условии своевременной диагностики и назначения верного лечения.
Лечение
Для лечения подбираются антибактериальные и противовоспалительные препараты в каждом конкретном случае. После проведения лечения в обязательном порядке назначаются препараты, обычно это пребиотики, направленные на восстановление микрофлоры. Если патологические изменения стали следствием воспалительного процесса в половых путях (бактериальной или вирусной), то назначается их санация с последующими мероприятиями по восстановлению нормальной вагинальной микрофлоры.
После проведения комплекса лечения и восстановительных мер необходимо продублировать осмотр с кольпоскопом. В тяжелых случаях для лечения реактивных состояний требуется более радикальное лечение с применением физических или химических методов воздействия. Исключением считается хирургическое вмешательство в виде конизации шейки матки.
МАЗОК НА ЦИТОЛОГИЮ — МЕТОД МИКРОСКОПИЧЕСКОГО ИССЛЕДОВАНИЯ ЦЕРВИКАЛЬНОГО ЭПИТЕЛИЯ С ЦЕЛЬЮ ПРЕДУПРЕЖДЕНИЯ И РАННЕЙ ДИАГНОСТИКИ РАКА ШЕЙКИ МАТКИ.
Мазок на цитологию в первую очередь проводится для обнаружения атипичных клеток, что позволяет на ранних этапах диагностировать дисплазию (CIN, LSIL, HSIL) или рак шейки матки. Это недорогой и удобный метод для профилактического охвата большого количества женщин. Конечно, чувствительность однократного исследования невысокая, однако ежегодный массовый скрининг в развитых странах позволил значительно снизить смертность женщин от рака шейки матки.
В связи с тем, что атипичные клетки могут располагаться на относительно небольшом участке слизистой, очень важно, чтобы материал был получен со всей поверхности шейки матки, особенно из цервикального канала! Для этого созданы специальные щеточки, позволяющие получать материал из недоступных для осмотра областей.
Особое внимание придается зоне трансформации, клетки которой чаще всего подвергаются опухолевому перерождению. Именно в зоне трансформации развивается до 80-90% рака шейки матки, остальные 10-20% приходятся на цервикальный канал.
Когда сдавать мазок на цитологию? Взятие мазка на цитологию следует проводить начиная с 5-го дня менструального цикла и за 5 дней до предполагаемого начала менструаций. Нельзя проводить анализ в течение двух суток после полового сношения либо введения во влагалище свечей. Несоблюдение этих правил может привести к ошибочной трактовке результатов. Так же наличие выраженного воспалительного процесса в шейке матки и влагалище серьезно осложняют диагностику.
Следует отметить, что забор материала — это достаточно неприятная процедура. Гинеколог должен соскоблить эпителий с поверхности шейки и зайти в цервикальный канал. Чем больше попадет эпителия из различных зон — тем качественнее диагностика. Иногда после цитологии могут оставаться кровоподтеки, это считается нормальным.
Таким образом, основное значение мазка на цитологию — это определение качественных изменений клеток. Для определения инфекционного агента, вызвавшего воспаление, лучше использовать мазок на флору или бактериологический посев. Однако при цитологическом исследовании врач может отметить присутствие каких либо микроогранизмов. К нормальной микрофлоре относятся палочки (лактобациллы), единичные кокки, в небольшом количестве может быть условно-патогенная флора. Наличие специфических инфекционных агентов (трихомонады, амебы, грибы, гонококки, гарднереллы, лептотрикс, хламидии, обилие кокков) считается патологией, которую необходимо лечить.
Обработка мазков. Сроки выполнения цитологии
После забора материала, образец переносится на предметное стекло, фиксируется и окрашивается. При прямом переносе мазка со щеточки возможна частичная потеря материала и деформация клеток, что ведет к снижению чувствительности метода и большому числу ложных результатов. На смену классическому методу пришла жидкостная цитология, что значительно повысило точность и качество исследования.
Жидкостная цитология — это новая технология обработки мазков, которая заключается в помещении проб в контейнер со специальным стабилизирующим раствором. При этом в раствор попадает весь полученный эпителий, который затем центрифугируется и очищается от нежелательных примесей (слизи и др). На сегодняшний момент жидкостная цитология становится «золотым стандартом» для исследования мазков со слизистой шейки матки. Но и в этом случае чувствительность однократного исследования не превышает 60-70%. В репродуктивном возрасте часты ложноотрицательные результаты, а у женщин в менопаузе — ложноположительные. Только трехкратное цитологическое исследование позволяет приблизиться к 100%.
Существуют различные методы окраски препаратов: по Papanicolau (Пап-тест), по Романовскому, по Wright-Diemsa, по Граму. Все методы направлены на окрашивание определенных клеточных структур, что позволяет дифференцировать различные типы эпителия, отличать клетки с ороговением и опухолевой трансформацией. Тест Папаниколау широко признан и сейчас используется как основной стандартизированный метод.
Сколько времени делается тест? В зависимости от организации процесса результат можно получить в течение 2-3 дней.
Цитограмма без особенностей — что это значит?
Варианты цитологического заключения широко варьируют. Как вариант нормы, могут употребляться следующие заключения: «цитограмма без особенностей«, «цитограмма в пределах нормы«, «цитограмма без интраэпителиальных поражений«, «цитограмма соответствует возрасту — атрофический тип мазка«, «NILM — Negative for intraepithelial lesion or malignancy«, «пролиферативный тип мазка«. Все это — НОРМА!
Слизистая шейки матки в норме гладкая, блестящая, влажная. Плоский эпителий бледно-розовый, железистый эпителий — ярко красный. Клеточный состав, который можно встретить в нормальной цитологии представлен в таблице.
Экзоцервикс | Хорошо сохранившиеся клетки плоского эпителия, преимущественно поверхностного, промежуточного слоев. |
Эндоцервикс | Клетки железистого (цилиндрического) эпителия. |
Зона трансформации | Клетки плоского эпителия, единичные клетки или мелкие скопления метаплазированного плоского эпителия, небольшие скопления железистого эпителия. |
Атрофический тип мазка — что это значит?
У женщин в переменопаузе и менопаузе за счет снижения общего уровня эстрогенов замедляются многие метаболические процессы, что приводит в результате к атрофии плоского эпителия. Эти изменения можно увидеть в цитограмме. Атрофический тип мазка относится к варианту нормальной цитограммы. Часто можно встретить в заключении фразу «цитограмма соответствует возрасту» или «возрастные изменения nilm«. Все это — варианты нормы!
Нужно понимать, что у женщин в менопаузе очень часты ложноположительные результаты цитограммы — тот случай, когда цитологу сложно отличить атрофичный плоский эпителий от дисплазии. Это нужно понимать, поскольку при последующей биопсии шейки матки патологии обычно не находят. Кроме того у пожилых женщин может быть склонность к кератинизации эпителия с формированием гиперкератоза (лейкоплакии).
Экзоцервикс | Хорошо сохранившиеся клетки плоского эпителия, преимущественно парабазального и базального слоев. Чаще бывают мазки атрофического типа, но могут быть и пролиферативного или смешанного типов. |
Эндоцервикс | Отсутствие клеток цилиндрического (железистого) эпителия не является показателем плохого качества мазка, поскольку в этот период зона трансформации смещается глубоко в канал и для получения железистого эпителия щеточку нужно вводить на глубину более 2-2,5 см. |
Зона трансформации | Клетки плоского, метаплазированного эпителия. |
Слизистая шейки матки в менопаузе истончена, легко травмируется и подкравливает, что является следствием снижения эстрогенов.
Расшифровка цитограммы
Терминология
Мазок на цитологию- норма
Дискариоз и дискариоциты — аномальные клетки с гиперхромными (плотными и темными) ядрами и нерегулярным ядерным хроматином. За дискариозом последует развитие злокачественного новообразования. Используется как синоним дисплазии, но как более общий термин.
Атипия — любое отличие структуры клетки от нормы. Смысл зачастую зависит от контекста. Но чаще все же используется для описания предопухолевых и опухолевых изменений.
Воспалительная атипия — сочетание дегенеративных, реактивных, пролиферативных изменений клеток при воспалении. Эти изменения могут стать причиной ложно-положительного диагноза дисплазии или рака.
Дисплазия — процесс нарушения созревания плоского эпителия. Является истинным предопухолевым процессом. Имеет 3 степени. К первой обычно относят вирусное поражение, ко второй и третьей — поражение с опухолевым потенциалом.
Дисплазия эпителия тяжелой степени
ASCUS — атипичные клетки, которые трудно дифференцировать с реактивной атипией и собственно предопухолевым процессом. Атипия неясного значения.
Дискератоз — нарушение кератинизации отдельных клеток плоского эпителия. Является признаком ВПЧ.
Паракератоз — нарушение кератинизации эпителиального пласта. Поверхностные клетки плоского эпителия всегда имеют некоторую степень кератинизации — это защитный механизм. Паракератоз может наблюдаться в норме, при раздражении слизистой любой причины, или при ВПЧ-поражении.
Койлоцитоз (койлоцитарная атипия, койлоцит) — специфические изменения ядер, характерные для вируса папилломы человека.
Койлоциты, многоядерная клетка
Гиперкератоз (лейкоплакия) — выраженная кератинизация эпителиального пласта с появлением защитного бесструктурного слоя из кератогиалина. Это нормальный процесс для кожи, но в слизистых оболочках считается патологией. Наблюдается при ВПЧ-инфекции, а также при раздражении слизистой, особенно при опущении органов малого таза, выпадении матки.
Плоскоклеточная метаплазия — защитный механизм, физиологический процесс замещения нежного железистого эпителия более устойчивым плоским эпителием. Метаплазированный эпителий часто становится источником дисплазии и рака, так как легко поражается вирусом папилломы человека.
Железистая гиперплазия — пролиферация, активный рост железистого эпителия. Является реактивным процессом при воспалении, эрозии шейки матки. Часто встречается при использовании гормональных препаратов.
Классификация Bethesda (США) — расшифровка цитограммы
Материал полноценный | Содержит клетки плоского и цилиндрического эпителия в достаточном количестве. |
Неудовлетворительный для оценки (неинформативный) материал | Скудное количество клеток или их отсутствие. |
Цитограмма в пределах нормы (NILM) | Содержит клетки поверхностного и промежуточного слоев многослойного плоского эпителия, клетки метаплазированного эпителия, лейкоциты, клетки цилиндрического эпителия, клетки эпителия эндометрия. |
Метаплазия (норма) | Клетки плоского метаплазированного эпителия свидетельствуют о том, что материал забран из зоны трансформации. |
Цитограмма воспаления | Дегенеративные и реактивные изменения клеток, воспалительная атипия, плоскоклеточная метаплазия, гиперкератоз, паракератоз, койлоцитоз и другие признаки вирусного поражения. |
Атрофия | Клетки базального и парабазального типов -мелкие клетки с гиперхромным ядром и скудной цитоплазмой. Часто могут ошибочно трактоваться как клетки с атипией, давая ложноположительный результат цитологии. |
ASCUS (atypical squmous cells of undetermined significance) | Изменения, которые трудно дифференцировать между реактивными изменениями эпителия и дисплазией. При ASCUS определяются клетки, трактовка которых затруднена — клетки с дискариозом, укрупненными и гиперхромными ядрами. Рекомендуется динамическое наблюдение и дообследование, а именно повторное цитологическое исследование через 6 месяцев и ВПЧ-тестирование. В случае подтверждении ASCUS и наличии вируса папилломы человека высокого онкогенного риска — проводится кольпоскопия. Исследования показывают, что 20% женщин с ASC имеют дисплазию после более тщательного обследования. |
LSIL (CIN I) | Слабовыраженное интраэпителиальное поражение, включающее папилломавирусную инфекцию. Рекомендуется наблюдение без активной терапии. У большинства женщин LSIL самостоятельно регрессирует в течение нескольких лет. В эту группу объеденены все изменения с низким злокачественным потенциалом, поскольку цитолог зачастую не может отличить изменения при ВПЧ инфекции и собственно CIN 1. |
HSIL (CIN I-II) | Умеренно выраженное и тяжелое интраэпителиальное поражение. Рекомендуется удаление всех пораженных тканей методом (конизация) с последующим морфологическим исследованием. В эту группу объеденены все изменения с высоким злокачественным потенциалом. |
AGC (atypical glandular cells) | Атипические клетки цилиндрического эпителия. Рекомендуется выскабливание цервикального канала для гистологического исследования. |
Плоскоклеточный рак | Злокачественная опухоль из плоского эпителия. |
Железистый рак | Злокачественная опухоль из железистого эпителия эндоцервикального типа. |
Эндометриальный рак | Злокачественная опухоль, развивающаяся из слизистой оболочки матки и прорастающая в цервикальный канал. |
Классификация цитологических изменений по Папаниколау несколько проще, чем Bethesda. Однако, посыл остается тем же. Имеется раздел доброкачественных реактивных изменений, которые не угрожают здоровью, класс предопухолевой патологии и собственно рак шейки матки. Каждый класс выделен на основании прогноза, риска злокачественной трансформации и необходимого медицинского вмешательства.
Цитограмма в гинекологии — это цитологическое исследование цервикального мазка, а если сказать проще — анализ «отпечатка» с шейки матки для определения состояния клеток ее поверхностного слоя — эпителия. Другое название анализа — онкоцитология или цитология. Выполняется с целью выявления предрака и рака шейки матки. Для справки: существует около 80 признаков атипичной (раковой) клетки, но в основном цитологами используется 10 из них.
Но иногда результат звучит для пациентки не совсем понятно — «цитограмма воспаления с реактивными или дегенеративными изменениями эпителия»… Что это значит и как лечится? Это 2 тип мазка по Папаниколау (Пап-тест), 2 класс — ASCUS — atypical squamous cell undetermined significance. Если говорить по-русски, то обнаружены клетки неопределенного значения, наиболее характерные для воспалительного процесса. Обычно при такой расшифровке еще встречается упоминание о значительном количестве лейкоцитов. То есть все что требуется от женщины — пройти противовоспалительное лечение, только не народными средствами. Какое именно? Зависит от инфекционных возбудителей и симптоматики.
Иногда одной цитограммы не достаточно, чтобы назначить лечение. И тогда врач берет мазок для культурального исследования. Это когда в лабораторных условиях «выращивают» микроорганизмы, находящиеся в мазке. Это занимает несколько дней.
Как лечить воспаленную шейку
Если это вагинальный кандидоз (молочница), то потребуется местное лечение противогрибковыми препаратами, например, «Клотримазолом». Также против молочницы используются лекарственные средства с действующими веществами: изоконазол, миконазол, натамицин, нистатин.
Если это бактериальный вагиноз, в мазках из цервикального канала обнаружены ключевые клетки, кокковая флора, то назначат антисептические средства вроде «Гексикона» (хлоргексидина), метронидазол или препарат комплексного действия — «Тержинан».
Если обнаружен урогенитальный трихомониаз назначаются препараты группы нитроимидазолов: Метронидазол, Тернидазол, Неомицин, Нистатин.
При хламидийном цервиците принимают тетрациклин, доксициклин и или эритромицин внутрь.
При актиномикозе, спровоцированном ношением внутриматочной спирали (ВМС), необходимо сначала удалить внутриматочный контрацептив. А далее, есть варианты. Это могут быть антибиотики пенициллинового ряда:
- тетрациклины;
- цефалоспорины (цефаклор, цефалексин);
- аминогликозиды (амикацин, гентамицин, тобрамицин).
Антибактериальные препараты широкого действия:
- метронидазол (метрогил, трихопол, эфлоран);
- клиндамицин (далацин, климицин, клиндамицин).
Отлично помогают следующие лекарственные средства:
- ко-тримоксазол;
- сульфадиметоксин;
- сульфакарбамид.
В случае обострения генитального герпеса, врачи рекомендуют провести местную терапию препаратами:
Если рецидивы заболевания частые, эти же лекарства назначаются в виде таблеток для приема внутрь.
К сожалению, лечения ВПЧ не существует. Но в течение 1-2 лет после манифестации заболевания иммунитет перебарывает вирус, и тот перестает негативно влиять на шейку. Главное, продержаться эти несколько месяцев.
При атрофическом кольпите (атрофический тип мазка, АТМ) лечение проводится антибактериальными средствами, обычно комплексного действия, а в дальнейшем женщине назначают заместительную гормональную терапию (ЗГТ) при необходимости и в случае отсутствия для нее противопоказаний.
Причины воспалительного типа мазка (ВТМ)
- Инфекции и грибковые заболевания:
- трихомониаз;
- кандидоз;
- хламидиоз;
- актиномикоз;
- генитальный герпес;
- ВПЧ.
- Атрофический вагинит.
- Лучевая терапия (поражение радиацией).
- Беременность.
- Воздействие некоторых медикаментов.
- Внутриматочная спираль (ВМС).
Может ли быть причиной отклонений не инфекционный процесс, а предрак или рак? Как мы уже ранее говорили, при таком виде заключения врачи называют клетки «неопределенными». Пока не совсем понятно — пройдет это самостоятельно после лечения или это все-таки предрак и необходимо удаление патологических участков шейки матки.
Мазок с шейки матки воспалительного типа (ВТМ) бывает и при легкой дисплазии (CIN 1). Ее наблюдают (женщина раз в 6 месяцев сдает мазок на онкоцитологию) или советуют удалить после биопсии — азотом, радиоволнами, электрическим током или иным способом.
Все что касается дисплазии, вы можете прочесть в этом материале.
Если цитологическое исследование, соскоб выявил у вас ВТМ, необходимо проконсультироваться у врача относительно санации (лечения инфекции) шейки. После этого, примерно через 3 месяца нужно повторить онкоцитологию (лучше жидкостную цитологию) и если мазок вновь не будет в норме (не nilm), назначается кольпоскопия и дальнейшее обследование.
Расшифровка терминов из цитограммы
Вы можете прочитать в своем заключении следующие термины.
Койлоциты — измененные клетки, характерны для ПВИ или ВПЧ (вируса папилломы человека), подробно о них мы писали здесь.
Нейтрофилы — разновидность лейкоцитов.
Дискератоциты — как и койлоциты являются признаком ПВИ.
Экзоцервикс — слизистая вагинальной части шейки матки.
Эндоцервикс — ткань (слизистая) непосредственно цервикального канала.
Гиперкератоз — ороговение эпителия шейки матки. Многие считают это предраковым состоянием, однако это не так. Если такое слово присутствует в вашей цитограмме, необходимо сделать кольпоскопию, и если врач найдет участок на шейке с предположительно атипичными клетками, то возьмет оттуда прицельную биопсию. Далее, лечение патологии проводится в зависимости от гистологического результата. В случае имеющегося воспалительного процесса, все это необходимо делать после лечения.
Лейкоциты — клетки в большом количестве указывающие на воспаление. В идеале, сначала врачи должны брать мазок на степень чистоты из влагалища и только потом цитологический, так как последний информативен именно при здоровой флоре.
Эритроциты — клетки крови. Если они есть в результатах вашего мазка, то это значит, при заборе материала был поврежден маленький сосудик. Когда слизистая шейки воспалена, такое нередко бывает.
Элементы воспаления — признаки воспаления.
Смешанная флора — в микрофлоре влагалища присутствуют как безобидные микроорганизмы, так и болезнетворные и условно-патогенные. При большом количестве лейкоцитов требуется лечение.
Палочковая флора — это абсолютная норма. Микрофлора влагалища в порядке.
Реактивный генез — бояться слова «реактивный» не стоит. Это не значит «быстрый», «скорый» и подобное. Это не рак и не предрак. Это синоним слова «реакция». То есть реакция клеток на инфекцию.
Эктопия — еще ее называют эрозией (что не верно), псевдоэрозией, эндоцервикозом, границей или зоной трансформации. Это стык двух видов эпителия — многослойного, расположенного на вагинальной части шейки матки или цилиндрического — клеток цервикального канала. На протяжении жизни женщины эта граница мигрирует и с возрастом уходит внутрь шейки.
Эндоцервицит — воспаление слизистой цервикального канала.
Атипия — клеточные изменения. Могут быть как при онкологии, так и воспалительных реакциях. ASCUS — это как раз означает наличие клеток неопределенного значения.
Анаэробные клетки — бактерии, вызывающие болезнь
Атрофический тип мазка — так проявляется недостаток эстрогенов в организме. Актуально для периода менопаузы. Слизистая шейки легко травмируется, тонкая, кровит.
Эстрогенный тип мазка — норма для женщин репродуктивного возраста.
HPV — вирус папилломы человека или папилломавирусная инфекция. При плохой цитограмме обычно выявляются ее онкогенные штаммы — ВПЧ 16 и 18 типа.
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